In the case study of stranded and prokaryotic RNAseq data, the Det'Rprok workflow detects candidates of 3 kinds of non coding RNA: 5'UTRs, antisense RNAs, and small RNAs.
Inputs: i) an mapping file (bam format) containing one valid alignment by read, ii) a feature file (gff format) annotating the genomic sequences used for the mapping.
Dependencies (from the Galaxy toolshed): "s_mart", "detrprok_scripts"
Preview
Import
Workflow Components
Inputs (16)
Name |
Description |
genome annotations |
Select here the GFF annotation file containing coding genes, tRNAs, rRNAs and known ncRNAs. You can download it from GJI (http://genome.jgi.doe.gov) or NCBI (http://ftp.ncbi.nih.gov/genomes/Bacteria). Caution: 1srt column should have exactly the same identifier as the genome sequence used for mapping. |
read alignments on genome |
Select here the bam file resulting from the mapping of RNA-seq reads on the genome sequence. You may use Bowtie or another mapper (use appropriate mapping option to retain only uniquely mapping reads). |
distance |
runtime parameter for tool clusterize |
OptionMin |
runtime parameter for tool restrict from size |
OptionMin |
runtime parameter for tool restrict from size |
optionType |
runtime parameter for tool clean transcript file |
OptionMin |
runtime parameter for tool select by tag |
OptionMin |
runtime parameter for tool select by tag |
distance |
runtime parameter for tool clusterize |
distance |
runtime parameter for tool clusterize |
OptionMin |
runtime parameter for tool restrict from size |
OptionMin |
runtime parameter for tool select by tag |
distance |
runtime parameter for tool clusterize |
distance |
runtime parameter for tool clusterize |
OptionDistance |
runtime parameter for tool compare overlapping small query |
OptionDistance |
runtime parameter for tool compare overlapping small query |
Steps (43)
Name |
Tool |
Description |
Input dataset |
None |
|
Input dataset |
None |
|
Concatenate datasets |
cat1 |
|
clusterize |
MergingDataClusterize |
|
remove introns |
removeIntrons |
|
collapse reads |
collapseReads |
|
compare overlapping small query |
CompareOverlappingSmallQuery |
|
compare overlapping small query |
CompareOverlappingSmallQuery |
|
splitTranscriptGff |
toolshed.g2.bx.psu.edu/repos/clairetn/detrprok_scripts/splitTranscriptGff/1.0.0 |
|
restrict from size |
restrictFromSize |
|
restrict from size |
restrictFromSize |
|
compare overlapping small reference |
CompareOverlappingSmallRef |
|
clean transcript file |
CleanTranscriptFile |
|
select by tag |
SelectByTag |
|
select by tag |
SelectByTag |
|
change tag name |
changeTagName |
|
clusterize |
MergingDataClusterize |
|
clusterize |
MergingDataClusterize |
|
Filter |
Filter1 |
|
Filter |
Filter1 |
|
Filter |
Filter1 |
|
remove introns |
removeIntrons |
|
remove introns |
removeIntrons |
|
collapse reads |
collapseReads |
|
change GFF features |
changeGffFeatures |
|
change GFF features |
changeGffFeatures |
|
change GFF features |
changeGffFeatures |
|
change tag name |
changeTagName |
|
change tag name |
changeTagName |
|
restrict from size |
restrictFromSize |
|
change GFF features |
changeGffFeatures |
|
change GFF features |
changeGffFeatures |
|
select by tag |
SelectByTag |
|
colorGff |
toolshed.g2.bx.psu.edu/repos/clairetn/detrprok_scripts/colorGff/1.0.0 |
|
clusterize |
MergingDataClusterize |
|
colorGff |
toolshed.g2.bx.psu.edu/repos/clairetn/detrprok_scripts/colorGff/1.0.0 |
|
change GFF features |
changeGffFeatures |
|
colorGff |
toolshed.g2.bx.psu.edu/repos/clairetn/detrprok_scripts/colorGff/1.0.0 |
|
clusterize |
MergingDataClusterize |
|
remove introns |
removeIntrons |
|
remove introns |
removeIntrons |
|
compare overlapping small query |
CompareOverlappingSmallQuery |
|
compare overlapping small query |
CompareOverlappingSmallQuery |
|
Outputs (41)
Name |
Type |
out_file1 |
input |
outputFileGff |
gff3 |
outputFile |
gff3 |
outputFileGff |
gff3 |
outputFileGff |
gff3 |
outputFileGff |
gff3 |
outputFile |
gff3 |
outputFileGff |
gff3 |
outputFileGff |
gff3 |
outputFileGff |
gff3 |
outputFile |
gtf |
outputFileGff |
gff3 |
outputFileGff |
gff3 |
outputFileGff |
gff3 |
outputFileGff |
gff3 |
outputFileGff |
gff3 |
out_file1 |
input |
out_file1 |
input |
out_file1 |
input |
outputFile |
gff3 |
outputFile |
gff3 |
outputFileGff |
gff3 |
outputFile |
gff |
outputFile |
gff |
outputFile |
gff |
outputFileGff |
gff3 |
outputFileGff |
gff3 |
outputFileGff |
gff3 |
outputFile |
gff |
outputFile |
gff |
outputFileGff |
gff3 |
outputFile |
gff |
outputFileGff |
gff3 |
outputFile |
gff |
outputFile |
gff |
outputFile |
gff |
outputFileGff |
gff3 |
outputFile |
gff3 |
outputFile |
gff3 |
outputFileGff |
gff3 |
outputFileGff |
gff3 |
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