Galaxy workflow for the identification of candidate genes clusters
Created: 2015-01-25 15:58:44
This approach screens two proteins against all nucleotide sequence from the NCBI nt database within hours on our cluster, leading to all organisms with an inter- esting gene structure for further investigation. As usual in Galaxy workflows every parameter, including the proximity distance, can be changed and additional steps can be easily added. For example additional filtering to refine the initial BLAST hits, or inclusion of a third query sequence.
https://github.com/bgruening/galaxytools/tree/master/workflows/ncbi_blast_plus/find_genes_located_nearby
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Workflow Components
Inputs (2)
Name |
Description |
Protein in FASTA format |
|
Protein in FASTA format |
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Steps (16)
Name |
Tool |
Description |
Input dataset |
None |
|
Input dataset |
None |
|
Cut |
Cut1 |
|
Compute |
toolshed.g2.bx.psu.edu/repos/devteam/column_maker/Add_a_column1/1.1.0 |
|
Compute |
toolshed.g2.bx.psu.edu/repos/devteam/column_maker/Add_a_column1/1.1.0 |
|
Cut |
Cut1 |
|
Intersect |
toolshed.g2.bx.psu.edu/repos/devteam/intersect/gops_intersect_1/0.0.1 |
|
Group |
Grouping1 |
|
NCBI BLAST+ tblastn |
toolshed.g2.bx.psu.edu/repos/devteam/ncbi_blast_plus/ncbi_tblastn_wrapper/0.1.01 |
|
NCBI BLAST+ tblastn |
toolshed.g2.bx.psu.edu/repos/devteam/ncbi_blast_plus/ncbi_tblastn_wrapper/0.1.01 |
|
Filter |
Filter1 |
|
Filter |
Filter1 |
|
Compute |
toolshed.g2.bx.psu.edu/repos/devteam/column_maker/Add_a_column1/1.1.0 |
|
Compute |
toolshed.g2.bx.psu.edu/repos/devteam/column_maker/Add_a_column1/1.1.0 |
|
Compute |
toolshed.g2.bx.psu.edu/repos/devteam/column_maker/Add_a_column1/1.1.0 |
|
Compute |
toolshed.g2.bx.psu.edu/repos/devteam/column_maker/Add_a_column1/1.1.0 |
|
Outputs (14)
Name |
Type |
out_file1 |
tabular |
out_file1 |
input |
out_file1 |
input |
out_file1 |
tabular |
output |
input |
out_file1 |
tabular |
output1 |
tabular |
output1 |
tabular |
out_file1 |
input |
out_file1 |
input |
out_file1 |
input |
out_file1 |
input |
out_file1 |
input |
out_file1 |
input |
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