Microbial Metagenomic Trait Statistical Analysis Workflow
Created: 2012-12-19 23:11:08
Last updated: 2014-11-12 09:45:08
This workflow retrieves the following microbial metagenomic traits from the Microbial Metagenomic Trait Database:
-GC content
-Variance of GC content
-Dinucleotides
-Number of rRNA
-Codon usage
-Amino acid composition
-Acidic to basic amino acids ratio
-% of Transcriptional factors
-% of classified reads
-Functional content
-Functional diversity
-Taxonomic content
-Taxonomic diversity
After the retrieval performs the ecological analyses described in Barberan et al. 2012 (http://dx.doi.org/10.1111/j.1365-294X.2011.05383.x)
Please read: https://wiki.biovel.eu/display/doc/Metagenomic+Workflows
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Authors (1)
Antonio Fernandez-Guerra, Pelin Yilmaz and Renzo Kottmann |
Titles (1)
Microbial Metagenomic Trait Statistical Analysis Workflow |
Descriptions (1)
This workflow retrieves the following microbial metagenomic traits from the Microbial Metagenomic Trait Database:
-GC content
-Variance of GC content
-Dinucleotides
-Number of rRNA
-Codon usage
-Amino acid composition
-Acidic to basic amino acids ratio
-% of Transcriptional factors
-% of classified reads
-Functional content
-Functional diversity
-Taxonomic content
-Taxonomic diversity
After the retrieval performs the ecological analyses described in Barberan et al. 2012 (http://dx.doi.org/10.1111/j.1365-294X.2011.05383.x)
Please read: https://wiki.biovel.eu/display/doc/Metagenomic+Workflows |
Dependencies (0)
Inputs (1)
Name |
Description |
mg_list |
A list or file of metagenomes present in the Microbial Metagenomic Trait Database (http://mb3is.megx.net/mg-traits)
Select the metagenomes and downlad the file list from http://blast2.mpi-bremen.de/mg-traits/samples
To run the workflow, at least select more than 10 metagenomes.
|
Processors (2)
Name |
Type |
Description |
retrieve_traits |
workflow |
|
Metagenomic_Traits_S |
workflow |
|
Outputs (19)
Name |
Description |
phyloMantel |
Mantel test against phylogenetic distance matrix (controlling for functional response)
|
funcMantel |
Mantel test against functional distance matrix (controlling for phylogenetic response)
|
summary_traits |
A table with all the traits used in this statistical analysis.
|
pcaLog |
Log file from the PCA analysis
|
traits_biplot |
PCA biplot.
|
bivariatePlot |
All against all trait realtionships.
|
traitsPCAPlot |
Plot of the PCA of all traits.
|
funcNMDSLog |
Log file from the functional NMDS
|
taxoNMDSLog |
Log file from the functional NMDS
|
funcNMDSTxt |
Data used for the functional NMDS plot
|
taxoNMDSPlot |
Plot of the taxonomic NMDS
|
funcNMDSPlot |
Plot of the functional NMDS
|
taxoNMDStxt |
Data used for the taxonomic NMDS plot
|
aa_content |
Aminco Acid content ot the metagenomes.
|
codon_usage |
Codon Usage data of the metagenomes.
|
di_nucleotide_odds_ratio |
Di-nucleotide odds-ration data of the metagenomes.
|
functional_content |
Functional content data of the metagenomes.
|
simple_traits |
Simple triats data of the metagenomes.
|
taxonomic_content |
Taxonomic content of the metagenomes.
|
Datalinks (26)
Source |
Sink |
mg_list |
retrieve_traits:mg_list |
retrieve_traits:dinuc |
Metagenomic_Traits_S:dinuc |
retrieve_traits:functional_content |
Metagenomic_Traits_S:func |
retrieve_traits:aa |
Metagenomic_Traits_S:aa |
retrieve_traits:simple_traits |
Metagenomic_Traits_S:traits |
retrieve_traits:taxonomic_content |
Metagenomic_Traits_S:phylo |
retrieve_traits:codon |
Metagenomic_Traits_S:codon |
Metagenomic_Traits_S:phyloMantel |
phyloMantel |
Metagenomic_Traits_S:funcMantel |
funcMantel |
Metagenomic_Traits_S:summary_traits |
summary_traits |
Metagenomic_Traits_S:pcaLog |
pcaLog |
Metagenomic_Traits_S:traitsBiplot |
traits_biplot |
Metagenomic_Traits_S:bivariatePlot |
bivariatePlot |
Metagenomic_Traits_S:traitsPcaPlot |
traitsPCAPlot |
Metagenomic_Traits_S:funcNMDSLog |
funcNMDSLog |
Metagenomic_Traits_S:phyloNMDSLog |
taxoNMDSLog |
Metagenomic_Traits_S:funcNmdsTxt |
funcNMDSTxt |
Metagenomic_Traits_S:phyloNmdsPlot |
taxoNMDSPlot |
Metagenomic_Traits_S:funcNmdsPlot |
funcNMDSPlot |
Metagenomic_Traits_S:phyloNmdsTxt |
taxoNMDStxt |
retrieve_traits:aa |
aa_content |
retrieve_traits:codon |
codon_usage |
retrieve_traits:dinuc |
di_nucleotide_odds_ratio |
retrieve_traits:functional_content |
functional_content |
retrieve_traits:simple_traits |
simple_traits |
retrieve_traits:taxonomic_content |
taxonomic_content |
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