User: Marc Ciosi

Name: Marc Ciosi

Joined: Friday 29 November 2019 17:13:33 (UTC)

Last seen: Monday 01 June 2020 22:04:17 (UTC)

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Website: https://www.gla.ac.uk/researchinstitutes/biology/staff/marcciosi/

Location: Glasgow, United Kingdom

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Galaxy

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Marc Ciosi

cutadapt+BWA-MEM-mis1-i2,2-gapex2,2+filter... (1)

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This workflow is designed to genotype trinucleotide repeats from sequencing reads that span the repeat and its flanksDetailed description as follows:1. Input fastq sequencing reads files (Galaxy tool: Input dataset)Input files for the workflow are single-end MiSeq reads (R1 produced using the protocol from Ciosi et al. 2018) or PacBio reads of insert (ROI) in fastq files format.2. Removing Illumina sequencing adaptor sequence in 3’ of the MiSeq reads (Galaxy tool: Cutadapt 1.16)Cutadapt...

Created: 2020-06-01 | Last updated: 2020-06-01

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