Pack: Delete unwanted DNA from plasmids
Title: Delete unwanted DNA from plasmids
Description
psg5 (for HEG0 ERa, EcoR1): https://www.lablife.org/pglabs?f=c&cmd=viewvecseq&vectorid=3768
1021 GTAATACGACTCACTATAGGGCG AATT
CGGATCCAGATCTTATTAAAGCA GAACTTGTTT
Forward: 5'-ATACGACTCACTATAGGGCGCGGATCCAGATCTTAT-3' (20, 16)
Reverse: 5'-TAATAAGATCTGGATCCGCGCCCTATAGTGAGTCG-3' (18, 17)
pGL2 (for p15 4xSBR1, kpn1, insert: 160 bp): https://www.lablife.org/pglabs?f=c&cmd=viewvecseq&vectorid=3345
1 cccgggaggtaccgagctcttacgcgtgct agctcgagat ctaagtaagc
5551 ccaaactcat caatgtatct tatggtactg taactgagct aacataa
Forward: 5'-gagctaacataacccgggagcgagctcttacgcgtg-3'
Reverse: 5'-gcacgcgtaagagctcgctcccgggttatgttagc-3'
pBABE-puro (for SHP1 WT, insert:2100 bp, EcoR1): https://www.lablife.org/pglabs?f=c&cmd=viewvecseq&vectorid=5484
1351 ggccggatcccagtgtggtggtacgtaggaattcgccagcacagtggtcgaccc
Forward: 5'-ccagtgtggtggtacgtaggcgccagcacagtggtc-3'
Reverse: 5'-tcgaccactgtgctggcgcctacgtaccaccacac-3'
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