Pack: Delete unwanted DNA from plasmids

Created: 2009-02-04 06:34:43      Last updated: 2009-02-04 15:47:23
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Title: Delete unwanted DNA from plasmids


Information Description

 

psg5 (for HEG0 ERa, EcoR1): https://www.lablife.org/pglabs?f=c&cmd=viewvecseq&vectorid=3768

1021 GTAATACGACTCACTATAGGGCG AATT CGGATCCAGATCTTATTAAAGCA GAACTTGTTT
Forward: 5'-ATACGACTCACTATAGGGCGCGGATCCAGATCTTAT-3' (20, 16)

Reverse: 5'-TAATAAGATCTGGATCCGCGCCCTATAGTGAGTCG-3' (18, 17)

 

pGL2 (for p15 4xSBR1, kpn1, insert: 160 bp): https://www.lablife.org/pglabs?f=c&cmd=viewvecseq&vectorid=3345

1 cccgggaggtaccgagctcttacgcgtgct agctcgagat ctaagtaagc
5551 ccaaactcat caatgtatct tatggtactg taactgagct aacataa

Forward: 5'-gagctaacataacccgggagcgagctcttacgcgtg-3'

Reverse: 5'-gcacgcgtaagagctcgctcccgggttatgttagc-3'

 

pBABE-puro (for SHP1 WT, insert:2100 bp, EcoR1): https://www.lablife.org/pglabs?f=c&cmd=viewvecseq&vectorid=5484

1351 ggccggatcccagtgtggtggtacgtaggaattcgccagcacagtggtcgaccc

Forward: 5'-ccagtgtggtggtacgtaggcgccagcacagtggtc-3'

Reverse: 5'-tcgaccactgtgctggcgcctacgtaccaccacac-3'

 

 

 

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