Pack Details
| Title | Delete unwanted DNA from plasmids |
| Items | 0 |
| Created by | Jyuh |
| Created at | 06:34:43 on Wednesday, 04 February 2009 |
| Last updated at | 15:47:23 on Wednesday, 04 February 2009 |
Description
psg5 (for HEG0 ERa, EcoR1) : https://www.lablife.org/pglabs?f=c&cmd=viewvecseq&vectorid=3768
1021 GTAATACGACTCACTATAGGGC
G
AATT
C
GGATCCAGATCTTATTAAAGCA GAACTTGTTT
Forward: 5'-ATACGACTCACTATAGGGC
G
CGGATCCAGATCTTAT
-3' (20, 16)
Reverse: 5'-TAATAAGATCTGGATCCG CGCCCTATAGTGAGTCG -3' (18, 17)
pGL2 (for p15 4xSBR1, kpn1, insert: 160 bp) : https://www.lablife.org/pglabs?f=c&cmd=viewvecseq&vectorid=3345
1 cccggga
g
gtac
c
gagctcttacgcgtgct agctcgagat ctaagtaagc
5551 ccaaactcat caatgtatct tatggtactg taactgagct aacataa
Forward: 5'-gagctaacataacccgggag cgagctcttacgcgtg -3'
Reverse: 5'-gcacgcgtaagagctcg ctcccgggttatgttagc -3'
pBABE-puro (for SHP1 WT, insert:2100 bp, EcoR1) : https://www.lablife.org/pglabs?f=c&cmd=viewvecseq&vectorid=5484
1351 ggccggatcccagtgtggtggtacgtag g aatt c gccagcacagtggtcgaccc
Forward: 5'-ccagtgtggtggtacgtagg cgccagcacagtggtc -3'
Reverse: 5'-tcgaccactgtgctggcg cctacgtaccaccacac -3'