This workflow uses one or more services that are deprecated as of 31st December 2012 (almost 12 years ago), and may no longer function. Show details...
Microarray CEL file to candidate pathways
This workflow takes in a CEL file and a normalisation method then returns a series of images/graphs which represent the same output obtained using the MADAT software package (MicroArray Data Analysis Tool) [http://www.bioinf.manchester.ac.uk/MADAT/index.html]. Also retruned by this workflow are a list of the top differentialy expressed genes (size dependant on the number specified as input - geneNumber), which are then used to find the candidate pathways which may be influencing the observed changes in the microarray data. By identifying the candidate pathways, more detailed insights into the gene expression data can be obtained.
NOTE - You will also need to install R and Rserv on your machine and install the libaries required by the R script into you R library directory (see for basic info: http://www.cs.man.ac.uk/~fisherp/rlib.html)
The example inputs for this workflow are as follows:
Samples1 = one or more CEL files for cross-correlating with Samples2 CEL files (new line separated): Liver_Day1_Mouse.CEL Liver_Day2_Mouse.CEL
Samples2 = one or more CEL files for cross-correlating with Samples1 CEL files (new line separated):
Kideny_Day1_Mouse.CEL Kidney_Day2_Mouse.CEL
geneNumber = the number of differentialy expressed gene to be returned above a given p-value, e.g. 20 arrayTypeAffy = the name of the Mouse AffyMetrix array used, e.g. mouse4302 path = the direct path to the CEL file location, e.g. C:/Microarray_Data/CEL_FILES/ - note the forward slashes NormalizationMethod = the type of normalisation to perfrom, e.g. rma, gcrma or mmgmos testMethod = e.g. limma, mmtest or pplr p-value = the p-value cut-off value for the array data, e.g. 0.05 foldChange = the fold change value for the microarray data, e.g. 1 (means greater than 1 or less than -1)
Preview
Run
Run this Workflow in the Taverna Workbench...
Option 1:
Copy and paste this link into File > 'Open workflow location...'
http://myexperiment.org/workflows/40/download?version=1
[ More Info ]
Workflow Components
Reviews (0)
Other workflows that use similar services (98)
Only the first 2 workflows that use similar services are shown. View all workflows that use these services.
Pathways and Gene annotations for QTL region (1)
Created: 2011-05-27 | Last updated: 2011-05-27
Credits: Paul Fisher
Comments (2)
Log in to make a comment